| SEPARUH AKHIR : 1 | | |
| Ked | Bib | Nama | Pasukan | Masa | * |
| 1 | GU | MSSD PASIR GUDANG | 1 | GU20 | ARISSA HAYRAH BINTI HAMDAN | | 2 | GU18 | LANA ALEXA JOSE LIM | | 3 | GU19 | EDA LIM YI ENN | | 4 | GU17 | NURUL LIYANA BINTI MUHAMMAD FITRI MICHAEL |
| PASIR GUDANG | 54.96 s | Q |
| 2 | KG | MSSD KLUANG | 1 | KG3 | SRIKANDI NURIY IZZ ZUYYIN BINTI MOHD SYAHID | | 2 | KG4 | QASEH RIANA DAFIRA BINTI MOHD | | 3 | KG5 | NUR AISYAH SUMAIYAH BINTI MUHAMMAD DANISH HAIKAL | | 4 | KG1 | NUR FARAH INSYIRAH TAN BINTI ABDULLAH |
| KLUANG | 55.83 s | Q |
| 3 | KU | MSSD KULAI | 1 | KU24 | QISYA EIRDEENA BINTI AHMAD SUHAIMI | | 2 | KU17 | SITI NURALIA AQILAH BINTI MOHD AZMI | | 3 | KU23 | NUR AISYAH SYAMIMI BINTI MOHD SYAMSYIR | | 4 | KU16 | JESSY LIM XI JIE |
| KULAI | 57.77 s | Q |
| 4 | KT | MSSD KOTA TINGGI | 1 | KT10 | PUTRI IFFAH YASSA`IRAH BINTI MOHAMAD YASSIRARAFAT | | 2 | KT11 | NUR NAJLAA NAQIBAH BINTI MOHD RAHMAD | | 3 | KT17 | NUR IMTINAN BINTI NORHISHAM | | 4 | KT16 | NUR ALESYA HAWANI BINTI MOHD HAMEZAN |
| KOTA TINGGI | 58.52 s | q |
| 5 | TK | MSSD TANGKAK | 1 | TK8 | NURUL AINA SHAKINA BINTI JAMALUDDIN | | 2 | TK1 | WARDINA FATHIYA BINTI MUHAMMAD FARID | | 3 | TK2 | NUR FATIHAH BINTI ABD RAZAK | | 4 | TK4 | LOH YI QIAO |
| TANGKAK | 58.76 s | q |
| |
| SEPARUH AKHIR : 2 | | |
| Ked | Bib | Nama | Pasukan | Masa | * |
| 1 | JB | MSSD JOHOR BAHRU | 1 | JB18 | ELYSHA GOO BINTI HARIS GOO ENG NANG | | 2 | JB4 | KRISH ASHREEN A/P KRISHNAN MAHESWARAN | | 3 | JB6 | IRIS SYUHADA BINTI SUHAIMI | | 4 | JB2 | BEH SIN HUI |
| JOHOR BAHRU | 55.58 s | Q |
| 2 | BP | MSSD BATU PAHAT | 1 | BP24 | TEE SHI JIA | | 2 | BP23 | QASEH NUR SYADIAH BINTI SULIMAN | | 3 | BP25 | SHARIFAH NURSAKINAH BINTI SYED ABDUL RAHMAN | | 4 | BP29 | ARISSA NAJWA BINTI MAHADIR |
| BATU PAHAT | 57.10 s | Q |
| 3 | MR | MSSD MUAR | 1 | MR12 | NUR QASEH IMAN BINTI SHAHROL NIZAM | | 2 | MR5 | HOW WEN XIN | | 3 | MR13 | NOR AMIRA AIREENA BINTI TASLIM | | 4 | MR11 | NUR DAMIA DARWIESYAH BINTI RADDIN |
| MUAR | 57.13 s | Q |
| 4 | PN | MSSD PONTIAN | 1 | PN6 | CHOW YOU AI | | 2 | PN5 | NUR DANEA BATRIESYA BINTI MD SAIFUAZHAR | | 3 | PN8 | SITI NURHUSNA BINTI HISHAMUDIN | | 4 | PN1 | YONG XIAO QING |
| PONTIAN | 59.30 s | |
| 5 | SG | MSSD SEGAMAT | 1 | SG15 | KISSHERA RAO A/P PRASAO RAO | | 2 | SG16 | NUR AQEELA HAZIQAH BINTI HAIRUL NIZAM | | 3 | SG23 | NUR FARAHIYATHUL AIN BINTI IBRAHIM | | 4 | SG18 | NURHIDAYAH BINTI ZAINAL |
| SEGAMAT | 60.05 s | |
| 6 | MG | MSSD MERSING | 1 | MG26 | NUR UMI UMAIROH BINTI ABDUL AZIZ | | 2 | MG23 | NURUL FATIN NAJWA BINTI ABDUL RAHIM | | 3 | MG21 | SITI NUR AQILAH BINTI ABD RASHID | | 4 | MG19 | MIA ZARA MOHAMMAD AZWAN BINTI ABDULLAH |
| MERSING | 60.29 s | |
