| SEPARUH AKHIR : 1 | | |
| Ked | Bib | Nama | Pasukan | Masa | * |
| 1 | KT | MSSD KOTA TINGGI | 1 | KT19 | NUR ALEEYA SYAFIRA BINTI MOHD ALZAPIR | | 2 | KT13 | NUR SYANAJIMAH BINTI MOHD HANAFI | | 3 | KT14 | NUR QURATUL INSYIRAH BINTI HASBULLAH | | 4 | KT12 | ROSNANI BINTI MAHAMAD |
| KOTA TINGGI | 54.16 s | Q |
| 2 | KU | MSSD KULAI | 1 | KU31 | NUR SYUHADAH AKMAL BINTI SHUKUR | | 2 | KU29 | NUR DAYANA BATRISYA BINTI ROHASHAH INDRA | | 3 | KU32 | HUDA HAIDA HISAMUDDIN BINTI ABDULLAH | | 4 | KU30 | NURUL NATASHA BINTI.ABDULLAH |
| KULAI | 54.44 s | Q |
| 3 | JB | MSSD JOHOR BAHRU | 1 | JB9 | KASHWEINA NAIR A/P UNNEETHARAN | | 2 | JB13 | ASHLYNN KAUR HONDELL | | 3 | JB19 | QAISARAH BINTI NUJUMUDDIN | | 4 | JB6 | KEYSHA WONG KAI XIN |
| JOHOR BAHRU | 54.83 s | Q |
| 4 | TK | MSSD TANGKAK | 1 | TK9 | TEE PEH NEE | | 2 | TK2 | TEE YING XI | | 3 | TK3 | NURUL LATIFAH BIN ZULKEFLI | | 4 | TK5 | ALYA ZULAIKHA BINTI AHMAD ZAMZURI |
| TANGKAK | 58.11 s | |
| 5 | SG | MSSD SEGAMAT | 1 | SG15 | LOW EE XUAN | | 2 | SG20 | NUR ALYA FARHANA BINTI AHMAD FARID | | 3 | SG21 | QANIA ARISYA BINTI MD FIRDAUS | | 4 | SG14 | NUR AINA AFRINA BINTI NORAZLI |
| SEGAMAT | 59.35 s | |
| |
| SEPARUH AKHIR : 2 | | |
| Ked | Bib | Nama | Pasukan | Masa | * |
| 1 | BP | MSSD BATU PAHAT | 1 | BP34 | KEE JIA XIN | | 2 | BP33 | NG ZHI YI | | 3 | BP29 | NURUL QIZTINA AWATIF BINTI MOHD EZUAN | | 4 | BP23 | CHUA JO VIANNE |
| BATU PAHAT | 53.43 s | Q |
| 2 | GU | MSSD PASIR GUDANG | 1 | GU3 | ALYA QAISARA BINTI MUHD SAUFI | | 2 | GU4 | NUR DAMIA DARWISYAH BINTI ROSLEE | | 3 | GU2 | NUR AFRINA BINTI ROHAIZAT | | 4 | GU1 | KANG XIN YEE |
| PASIR GUDANG | 54.18 s | Q |
| 3 | KG | MSSD KLUANG | 1 | KG23 | DYNA QAISARA BINTI MOHD SUPIAN | | 2 | KG22 | HANNA SOH | | 3 | KG24 | NUR DAMIA MIRZA BINTI MOHD SYAHID | | 4 | KG21 | SIM SHU YUAN |
| KLUANG | 54.79 s | Q |
| 4 | MG | MSSD MERSING | 1 | MG39 | NURUL KARISMA BINTI RAZALI | | 2 | MG34 | SITI NUR AIN BINTI SHEK NASYARATUL NAIM | | 3 | MG37 | FELYEEYEN FENDIE | | 4 | MG32 | SAHIRA BINTI ABDUL RAZAK |
| MERSING | 55.89 s | q |
| 5 | PN | MSSD PONTIAN | 1 | PN39 | NUR AFIQAH SYAZANA BINTI MOHAMAD HALUAT | | 2 | PN28 | SEALIYANA BT MOHD RAZI | | 3 | PN27 | NUR DANIA EISYA BINTI ABD SHUKOR | | 4 | PN21 | NUR FARISHA NAJWA BINTI MOHAMAD ARIF |
| PONTIAN | 56.78 s | q |
| 6 | MR | MSSD MUAR | 1 | MR12 | PUTERI DAMIA HUMAIRAH BINTI NURUL ISKANDAR SHAH | | 2 | MR4 | NURALEEYA MAYSARAH BINTI MOHD BASRI | | 3 | MR6 | TEE KAI EN | | 4 | MR3 | TAN YING NING |
| MUAR | 58.51 s | |
